RSP5 Positively Regulates the Osteogenic Differentiation of Mesenchymal Stem Cells by Activating the K63-Linked Ubiquitination of Akt

Figure 6

RSP5 induced the K63-linked ubiquitination of Akt. (a) Coimmunoprecipitated mixtures were separated by SDS-PAGE and evaluated by western blots. Endogenous RSP5 and Akt in MSCs interact with each other. (b) Myc-RSP5 significantly induced the ubiquitination of Flag-Akt. (c) Myc-RSP5 significantly induced the K63-linked ubiquitination of Flag-Akt. (d) Myc-RSP5 did not affect the K48-linked ubiquitination of Flag-Akt.

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