Phosphodiesterase 10A Is a Mediator of Osteogenic Differentiation and Mechanotransduction in Bone Marrow-Derived Mesenchymal Stromal Cells

Table 1

Primer names, sequences, product lengths, annealing temperatures, and GenBank Accession numbers are shown.

Gene name	Primer	Sequence 5-3	Product length	Annealing temp (°C)	GenBank accession number
Primers for human genes
ALPL	ALPL_FOR	GTACGAGCTGAACAGGAACAACG	151	58	NM_000478.5
	ALPL_REV	CTTGGCTTTTCCTTCATGGTG
RPS27A [37]	RPS27A_FOR	TCGTGGTGGTGCTAAGAAAA	141	60	NM_001135592
	RPS27A_REV	TCTCGACGAAGGCGACT
RUNX2	Runx2_FOR	CTTCACAAATCCTCCCCAAG	147	58	NM_001024630.3
	Runx2_REV	ATGCGCCCTAAATCACTGAG
SPP1	SPP1_FOR	TATGATGGCCGAGGTGATAG	133	60	NM_001040058.2
	SPP1_REV	CATTCAACTCCTCGCTTTCC
Primers obtained from Qiagen
PDE10A	Hs_PDE10a_1_SG	Qiagen sequence			NM_001130690; NM_006661
PTGS2	Hs_PTGS2_1_SG	Qiagen sequence		59	NM_000963
FOS	Hs_FOS_1_SG	Qiagen sequence		57	NM_005252.3
Primers for murine genes
B2m	mB2m_FOR	GTCTTTCTGGTGCTTGTCTC	117	57	NM_009735.3
	mB2m_REV	AGTTCAGTATGTTCGGCTTC
Pde10a	mPDE10A_FOR	TCTGAAAGTGTTAGTGCAGAGA	98	57	NM_001290707.1
	mPDE10A_REV	TGGTACCTGCTGACTTCCTT
	mSPP1_REV	CGCTCTTCATGTGAGAGGTG