Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout

Figure 3

Screening data GO analysis. (a, b) Comparison of input 1 with input 2 for genome-scale negative selection screening. The most significantly depleted genes in mESCs transfected with both GeCKO (v2.0) library A and B. (c, d) GeCKO screen in mESCs reveals genes whose loss confers commitment resistance. The most significantly enriched genes including two positive controls Trp53 and Tcf7l1 (ranking first and second), as well as another 70 candidate genes (A/B-2i/LIF: E-group with library A/B).

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