Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout

<div>RT-qPCR analysis of the three pluripotency factors’ expression in wild-type OG2 mESCs transfected with lenti-Cas9-sgRNA of (a) Tcf7l1, (b) p53, (c) Jarid2, and (d) Fbxw7 and treated by withdrawing 2i/LIF. 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Stem Cells International

fig4

Figure 4

Figure 4: Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout 