Research Article
Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout
Table 1
Details of primers used for the qRT-PCR analysis of selected genes used in the PCR experiments.
| | Gene name | Primer sequences | Annealing T (°C) | GenBank accession number |
| | Gapdh | F: 5-AACTTTGGCATTGTGGAAGGGCTCA-3
R: 5-TTGGCAGCACCAGTGGATGCAGGGA-3 | 60 | NM_001289726.1 |
| | Nanog | F: 5-CTCAAGTCCTGAGGCTGACA-3
R: 5-TGAAACCTGTCCTTGAGTGC-3 | 60 | NM_028016.3 |
| | Esrrb | F: 5-TTTCTGGAACCCATGGAGAG-3
R: 5-AGCCAGCACCTCCTTCTACA-3 | 60 | NM_011934.4 |
| | Dppa5a | F: 5-GAAGTATTCCAGGTCCAGTC-3
R: 5-TTGAGAGCCGTAAATGAAGA-3 | 60 | NM_025274.3 |
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