Differentiation of Human Embryonic Stem Cells into Neuron, Cholinergic, and Glial Cells

Figure 2

Patch-clamp analysis of electrophysiological properties was performed on neuron. (a) Patched neuron, red arrow indicates the patch pipette while the patched cell is by the green arrow. White triangles indicate the processes of the patched cell. (b) An action potential (upper trace) is elicited by depolarizing current stimulation (pulse duration: 500 ms, range: -10-300 pA, increment: 10 pA). (c) Patched cholinergic neuron with red arrows indicating pipette and green arrow pointing at patched cell. White arrows indicate processes of the patched neuron. (d) Action potential elicited from the patched cholinergic neuron using the same settings as for neurons in (b).

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