Differentiation of Human Embryonic Stem Cells into Neuron, Cholinergic, and Glial Cells

Figure 5

Terminal differentiation cell cultures of neurons, oligodendrocytes, astrocytes, and cholinergic neurons were analyzed using qRT-PCR. In addition, the mixed neuronal cultures were analyzed for neuronal markers. Relative mRNA expressions of different targets were analyzed in the different cultures: (a) mean β-tubulin III expression in neurons , oligodendrocytes , astrocytes , and cholinergic ; (b) mean GFAP expression in neurons , oligodendrocytes , astrocytes , and cholinergic ; (c) mean GALC expression in neurons , oligodendrocytes , astrocytes , and cholinergic ; and (d) mean ChAT expression in neurons , oligodendrocytes , astrocytes , and cholinergic . (e) The neuronal culture was tested for several markers. mRNA expression of VGULT mean , VIAAT, ChAT, and β-tubulin III shows mixed culture of glutamatergic, GABA:ergic, and cholinergic neurons. Experiments were performed on two biological and three technical replicates of each lineage. Expression of each gene was normalized against RPL19.

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