Differentiation of Human Embryonic Stem Cells into Neuron, Cholinergic, and Glial Cells

Table 1

Details of primary antibody used for fluorescent immunocytochemistry.

Cell lineage	Antibody	Dilution ratio	Source	Cat. No.
NSC	Sox2	1 : 200	Invitrogen	A24354
	Nestin	1 : 200	Abcam	ab6142
	OCT4	1 : 200	Invitrogen	701756
Neurons	β-Tubulin III	1 : 200	Abcam	ab18207
Oligodendrocytes	GALC	1 : 200	Abcam	ab232972
Astrocytes	GFAP	1 : 1000	Millipore	MAB360
Cholinergic neurons	ChAT	1 : 100	Millipore	AB144P