Differentiation of Human Embryonic Stem Cells into Neuron, Cholinergic, and Glial Cells

Table 2

Primers used for qRT-PCR analysis of cultures. : annealing temperature in °C of primers.

Genes	Sequences 5 to 3 forward	Sequences 5 to 3 reverse
RPL19	TGAGGAGAATGAGG	GTACAGGCTGTCAT	55
β-Tubulin III	GGCATCTCTTGAGA	GACCTGTACCTGTC	55
GALC	GCCAAGCGTTACCATGATTT	TTTCACTCGCTGGAGACCTT	61.4
GFAP	CCCTCTGGAGAG	TCCTCCTCGTGGATCTTC	55.1
ChAT	AGATGTTCTGCTGC	GAAAAGGATGGTCTCTGG	55.8
VIAAT	CTCGGGTCCTTCTGTCCTT	ATCTTGGCCTGGGACTTGTT	61.4
VGLUT	GCCAAGCGTTACCATGATTT	TTTCACTCGCTGGAGACCTT	59