Pantoprazole (PPZ) Inhibits RANKL-Induced Osteoclast Formation and Function In Vitro and Prevents Lipopolysaccharide- (LPS-) Induced Inflammatory Calvarial Bone Loss In Vivo

Figure 2

PPZ attenuates podosomal-actin ring formation and bone resorption in vitro. (a) BMMs were stimulated by RANKL at 6 and 12.5 μM of PPZ and stained with podosomal-actin and DAPI, respectively (magnification: 100x; ). (b) BMMs were cultured on bone slices and stimulated by M-CSF and RANKL in the presence of different concentrations of PPZ. After 15 days, bone resorption lacunae were observed by scanning electron microscopy (magnification: 100x; ). (c, d) Quantitative measurement of the size and number of podosomal-actin ring per well using ImageJ. (e) Resorption pit area measurements. The area of bone resorption was quantitated using ImageJ. Data are presented as ; , relative to RANKL-induced controls.

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