Effects of DMSO on the Pluripotency of Cultured Mouse Embryonic Stem Cells (mESCs)

Figure 1

A small amount of DMSO has minimal effects in mESC culture. mESCs were cultured in two different media that mimic the naive pluripotency state and were kept in culture for 48 hours in the presence or absence of DMSO (control conditions—2i, FBS; DMSO-incubated conditions—0.0001% DMSO, 0.1% DMSO, 0.1001% DMSO, and 0.2% DMSO). (a) Schematic representation of the DMSO conditions used. (b) Representative images of plated cells acquired from randomly selected fields using phase contrast microscopy at the 48 h time point (100x magnification). (c) Total number of cells per well, 24 and 48 hours after treatment, from at least five independent experiments. (d) Daily growth rate analysis calculated using the total number of cells counted in each time point. Results are expressed as . Statistical significance considered when , , and .

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