Research Article

The m6A Methylation-Regulated AFF4 Promotes Self-Renewal of Bladder Cancer Stem Cells

Figure 1

Differential m6A levels between CSCs and non-CSCs of BCa. (a) Representative gating scheme for FACS sorting of ALDH1-stained 5637 and UM-UC-3 cells. (b) Quantification of m6A levels in ALDH1-positive and ALDH1-negative BCa cells (, , Student -test). (c) mRNA levels of RNA m6A writers and erasers in ALDH1-positive and ALDH1-negative BCa cells (, Student -test). (d) Protein levels of RNA m6A methyltransferase METTL3 in ALDH1-positive and ALDH1-negative BCa cells.
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