Research Article
The m6A Methylation-Regulated AFF4 Promotes Self-Renewal of Bladder Cancer Stem Cells
Figure 2
METTL3 is required to sustain self-renewal of BCa cells. The knockdown effect of specific siRNAs (si-METTL3-1 and si-METTL3-2) in 5637 and UM-UC-3 cells was verified at both the mRNA ((a) by qRT-PCR) and protein levels ((b) by Western blot). Ratio of cells with high ALDH activity (c, d); number and size of spheres formed in stem cell medium (e, f) of the BCa cells transfected with indicated siRNAs are plotted, and representative images are presented. compared to the scramble group, by Student -test. Scale bar, 250 μm.
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