The m<sup>6</sup>A Methylation-Regulated AFF4 Promotes Self-Renewal of Bladder Cancer Stem Cells

<div><i>AFF4</i> mimic the phenotype of <i>METTL3</i> in regulating the stemness of BCSCs. The knockdown effect of specific siRNAs (si-<i>AFF4</i>-1 and si-<i>AFF4</i>-2) in 5637 and UM-UC-3 cells was verified at both the mRNA ((a) by qRT-PCR) and protein levels ((b) by Western blot). Ratio of cells with high ALDH activity (c, d); number and size of spheres formed in stem cell medium (e, f) of the BCa cells transfected with indicated siRNAs are plotted, and representative images are presented. <span class="nowrap"><svg height="13.532pt" id="M16" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 50.6052 13.532" width="50.6052pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 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-3Z"></path></g><g transform="matrix(.013,0,0,-0.013,34.265,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,40.505,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,43.469,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,49.709,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> compared to the scramble group, by Student <span class="nowrap"><svg height="8.02022pt" 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Scale bar, 250 <i>μ</i>m.</div>

Stem Cells International

fig4

Figure 4

Figure 4: The m<sup>6</sup>A Methylation-Regulated AFF4 Promotes Self-Renewal of Bladder Cancer Stem Cells 

Stem Cells International

The m6A Methylation-Regulated AFF4 Promotes Self-Renewal of Bladder Cancer Stem Cells

Figure 4

The m⁶A Methylation-Regulated AFF4 Promotes Self-Renewal of Bladder Cancer Stem Cells