Research Article

The m6A Methylation-Regulated AFF4 Promotes Self-Renewal of Bladder Cancer Stem Cells

Figure 5

AFF4 regulates SOX2 and MYC expression in BCa cells. (a) ChIP assay showed the recruitment of AFF4 at MYC and SOX2 promoter regions in 5637 and UM-UC-3 cells transfected with indicated siRNAs at 48 h posttransfection. Expression of MYC and SOX2 in 5637 and UM-UC-3 cells transfected with indicated siRNAs (si-AFF4-1 and si-AFF4-2) was verified at both the mRNA ((b) by qRT-PCR) and protein levels ((c) by Western blot). , relative to the scramble group by Student -test.
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