The m⁶A Methylation-Regulated AFF4 Promotes Self-Renewal of Bladder Cancer Stem Cells

Figure 6

AFF4 is essential for BCa tumor propagating in vivo. Graph (a) and quantification (b) of the percentage of tumor-free mice 30 days after subcutaneous injection of different dilutions of AFF4 knockdown 5637 cells or control cells into immunodeficient mice ( for each dilution). (c) Ratio of ALDH-positive cells from the xenografts. by Student -test. (d) Quantitative measurement and representative images of AFF4, SOX2, and MYC expression in xenografts generated by AFF4 stable knockdown BCa cells and control cells. ( by Student -test). Scale bar, 50 μm. (e) Correlation between METTL3, AFF4, SOX2, and MYC mRNA expression and survival of BCa patients in TCGA dataset. Disease-free or overall patient survival in groups of high and low expression was analyzed by the Kaplan-Meier survival curve and compared by the log-rank test.

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