Preventive Effects of Intrauterine Injection of Bone Marrow-Derived Mesenchymal Stromal Cell-Conditioned Media on Uterine Fibrosis Immediately after Endometrial Curettage in Rabbit

Figure 3

Characterization of rabbit bone marrow-derived mesenchymal stromal cells (BM-MSCs). (a) Fibroblast-like and spindle-shaped morphology of rabbit BM-MSCs, a typical characteristic of MSCs in passage 3 (×400). (b) BM-MSCs of rabbits cultivated in osteogenic medium and stained with Alizarin red (×100). (c) BM-MSCs of rabbits cultivated in adipogenic medium and stained with oil red O at day 21 after induction (×200). (d) Agarose gel electrophoresis of products of reverse transcriptase polymerase chain reaction (RT-PCR) of BM-MSCs revealing to be positive for CD73 (mesenchymal marker) and negative for CD34 and CD45 (hematopoietic markers).

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