Low-Intensity Pulsed Ultrasound Modulates RhoA/ROCK Signaling of Rat Mandibular Bone Marrow Mesenchymal Stem Cells to Rescue Their Damaged Cytoskeletal Organization and Cell Biological Function Induced by Radiation

Figure 2

Radiation disrupted the cytoskeleton arrangement and vinculin distribution in M-BMMSCs. The cells were divided into three groups: the control group (a) that received no treatment; the radiation group (b) that was irradiated with 4 Gy of X-ray for 12 h; and the inhibitor group (c) that was subjected to Y-27632 30 min before radiation. Then, the cells were immunostained with F-actin (A1, B1, and C1) and counter stained with TRITC-vinculin (A2, B2, and C2) and DAPI (A3, B3, and C3). Graphs A4, B4, and C4 are the merged images. The white arrowheads represent the change in the cytoskeleton. The yellow arrowheads represent the change in vinculin distribution. Scale bars 50 μm.

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