Adipose Tissue-Derived Stromal Cells Induce a Highly Trophic Environment While Reducing Maturation of Monocyte-Derived Dendritic Cells

Figure 1

Experimental overview. Monocytes were isolated based on CD14 expression and induced to differentiate into immature dendritic cells by IL-4 and GM-CSF. Following 6 days of differentiation, small irregular dendrites were present. At this point, cocultures with ASC were established and allowed to recover for 1 day. The cocultures were matured by stimulation with LPS and IFN-γ for 2 days, supernatants were harvested and sampled for IL-12p70 and IL-10, and mature dendritic cells were analyzed for surface markers CD11c, HLA-DR, CD80, CD86, CD40, CD83, and PD-L1.