The Histone Demethylase KDM3B Promotes Osteo-/Odontogenic Differentiation, Cell Proliferation, and Migration Potential of Stem Cells from the Apical Papilla

Figure 2

KDM3B enhanced the cell proliferation of SCAPs and regulated the cell cycle. The (a) CFSE assay and (b) quantitative analysis results revealed that KDM3B knockdown decreased the cell numbers at 3 days in SCAPs. (c) The CCK-8 assay revealed that KDM3B knockdown reduced the cell numbers at 24 h and 48 h in SCAPs. (d) Flow cytometric cell cycle analysis revealed that KDM3B knockdown increased the proportion of cells in the G0/G1 phase and decreased the proportion of cells in the S phase. (e) Comparison of cell cycle distribution between the KDM3B knockout group and the control group. (f) The proliferation index between the KDM3B knockout group and the control group. The (g) CFSE assay and (h) quantitative analysis results revealed that KDM3B overexpression enhanced the cell numbers at 3 days in SCAPs. (i) The CCK-8 assay revealed that KDM3B overexpression increased the cell numbers at 24 h and 48 h in SCAPs. (j) Flow cytometric cell cycle analysis revealed that KDM3B overexpression decreased the percentage of cells in the G0/G1 phase and increased the proportion of cells in the S phase. (k) Comparison of cell cycle distribution between the KDM3B overexpression group and the control group. (l) The proliferation index between the KDM3B overexpression group and the control group. Statistical significance was determined using Student’s -test. All error bars represent SD ( or 6). . .

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