Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions

<div>qPCR assessment of relative gene expression in MSCs under normoxic and hypoxic conditions with the 50% or 100% NPC conditioned medium. Gene expression levels for each sample were normalized against the housekeeping gene <i>β</i>-actin. (a) Aggrecan: (i) normoxia, (ii) hypoxia; (b) brachyury: (i) normoxia, (ii) hypoxia; (c) collagen II: (i) normoxia, (ii) hypoxia; (d) KRT8: (i) normoxia, (ii) hypoxia; (e) KRT18: (i) normoxia, (ii) hypoxia; (f) KRT19: (i) normoxia, (ii) hypoxia; and (g) Shh: (i) normoxia, (ii) hypoxia. <span class="nowrap"><svg height="13.532pt" id="M40" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 50.6052 13.532" width="50.6052pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 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Stem Cells International

fig4

Figure 4

Figure 4: Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions 

Figure 4 | Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions 