Research Article
Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions
Table 1
Primer sequences for gene expression analysis used in qPCR.
| | Genes | Forward (5-3) | Reverse (5-3) |
| | HIF-1α | GCAACTGCCACCACTGATGA | GCTGTCCGACTGTGAGTACC | | HIF-2α | GCACCAGCAGTTCACACTTG | CTGACGGTCTTGTCAGGCAT | | Glut-1 | GCTGTGGCTGGCTTCTCTAA | CCGGAAGCGATCTCATCGAA | | Shh | TATGAGGGTCGAGCAGTGGA | AGTGGATGCGAGCTTTGGAT | | Brachyury | TCTAGGCACAACATGGCAGG | AAATTGGACCACAGCCTCGT | | Aggrecan | TGGCCTGCCTGACTTTAGTG | CCTGAACCACTGACGCTGAT | | Collagen II | GGCCAGGATGCCCGAAAATTA | ACCCCTCTCTCCCTTGTCAC | | Carbonic anhydrase 3 | TGGTTCACTGGAACCCGAAG | GAGCCTCCTTGCCCTTAGTC | | Carbonic anhydrase 12 | TCCGACAAGGACTGCTTACC | GGAGATCCCAAGGACACCAG | | CD24 | AGTAATTGACACGGGCCAGG | TGGGAAATGCTTTGCCGTTG | | Cytokeratin 8 | CCCGGCTTCAGCTATGGAAT | AGCCTTGGTGCGGCTATAAG | | Cytokeratin 18 | GACCCAGGAATACGAAGCCC | ACTTTGCCATCCACGACCTT | | Cytokeratin 19 | GAGATCGCCACCTACCGAAG | GGAAGGGCTGGTGTGAACTT |
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qPCR: quantitative polymerase chain reaction.
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