Reconstruction of Alzheimer’s Disease Cell Model In Vitro via Extracted Peripheral Blood Molecular Cells from a Sporadic Patient

Figure 2

The results of RT-PCR and teratoma formation. The expressions of Oct4, Sox2, Nanog, Rexl, Lin28, and other genes were identified by RT-PCR. Sox2, Rex1, Oct4, Nanog, and Lin28 gene of two strains of cells were positive in agarose gel electrophoresis, which was similar to H9 cells, but negative in primary PBMC (a). The formation of teratoma was observed in vivo, and its ability of multidirectional differentiation was tested. iPSCs were tested for tumorigenesis in nude mice. The two iPSCs had the potential to differentiate into three layers (b).

(a)

(b)