KIF2C Is a Novel Prognostic Biomarker and Correlated with Immune Infiltration in Endometrial Cancer

Figure 3

Anti-PD1 immunotherapy enhances the response to inhibit EC cells in vitro. (a) Representative images of KIF2C (green) and PDL1 (pink) in tumor tissues and Ishikawa cells were analyzed by multiplex fluorescent IHC (original magnification at ×200). (b) The correlations between KIF2C and PD-L1 in human EC and Ishikawa cells. Results were expressed as log (ln) of integrated density in EC tissues and Ishikawa cells. (c–f) After being transfected with shNC or shKIF2C, Ishikawa and RL95-2 were cocultured for 3 days with activated CD8⁺ T cells, in the presence or absence of anti-PD1. Representative data and statistical analysis of T cell apoptosis were shown (). (g, h) The LDH released in cell cocultured supernatant was measured at the indicated time (). Scale bars, 50 μm; error bars .

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