Adipose Mesenchymal Stem Cell-Derived Exosomes Enhance PC12 Cell Function through the Activation of the PI3K/AKT Pathway

Figure 3

ADSC-exo promote proliferation of PC12 cells. (a) PC12 cells were incubated with various concentrations of ADSC-exo (0, 10, 50, and 100 μg/ml) for 48 h, and cell proliferation was determined by CCK-8 assay. (b, c) PC12 cells were treated with various concentrations of ADSC-exo (0, 10, 50, and 100 μg/ml) for 24 h, and then, the cells were incubated with EdU for 3 h before fixation and stained. EdU-positive cells are expressed as percentages of positively stained cells compared with total stained cells per field. ADSC-exo: exosomes derived from adipose mesenchymal stem cells; CCK-8: Cell Counting Kit-8; EdU: 5-ethynyl-20-deoxyuridine. ; ; ; .

(a)

(b)

(c)