A Novel High-Content Screening-Based Method for Anti-Trypanosoma cruzi Drug Discovery Using Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Figure 1

Cardiac differentiation induction in hiPSC and characterization of hiPSC-CMs. (a) Schematic experimental design. (b) Flow cytometry analysis of hiPSCs at D(-3), before plating for cardiac induction, for TRA-1-60 expression, and hiPSC-CM purity at differentiation D14 evaluated by cardiac troponin-T (cTNT) expression. Light grey histograms represent isotype controls. (c) Representative phase contrast micrographies of the different days postinduction of cardiac differentiation from hiPSCs. . (d) Representative confocal microscopy images of hiPSC-CMs stained with sarcomeric alpha-actinin, sarcomeric myosin (MF-20), cardiac troponin T (cTNT), and GATA-4 (all in red). Nuclei were stained with DAPI (blue). . (e) Gene expression analysis by RT-qPCR demonstrating mRNA expression of cardiac genes NKX2.5, GATA-4, and TNNT2, normalized to the levels of GAPDH. ; .

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