Microenvironment Influences on Human Umbilical Cord Mesenchymal Stem Cell-Based Bone Regeneration

Figure 2

Characteristics of hUC-MSCs. (a) Umbilical cord. (b) Tissue explant culture of hUC-MSCs (). (c) Phase 3 hUC-MSCs on day 1 of culture (). (d) Phase 3 hUC-MSCs on day 4 of culture (). (e) Proliferation of hUC-MSCs was evaluated using a CCK-8 assay (, ). (f) Phenotype of hUC-MSCs. Green line indicates isotype control; purple-shaded histograms indicate positive staining. (g) Alizarin red staining on day 21 of culture (). (h) Oil red O staining on day 21 of culture (control group , induced group scale ).