Research Article
p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation
Figure 2
p57 suppressed the pluripotency state of mESCs. (a) Real-time PCR analyses of Nanog, Oct4, and Sox2 expression levels in control () and p57 knockdown (shp57, ) mESCs. (b, c) Western blot and related densitometric analyses of NANOG, OCT4, and SOX2 protein levels in control and p57 knockdown (shp57) mESCs cultured for 48 h. (d) Real-time PCR analyses of Nanog, Oct4, and Sox2 expression levels in control () and p57-overexpressing (p57, ) mESCs cultured for 48 h. (e, f) Western blot and related densitometric analyses of NANOG, OCT4, and SOX2 protein levels in control and p57-overexpressing (p57) mESCs cultured for 48 h. , , and .
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