Research Article

p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation

Figure 4

p57 suppressed the proliferation of mESCs. (a) The growth curve of control () and p57 knockdown (shp57, ) mESCs. (b, c) EdU staining results of control () and p57 knockdown (shp57, ) mESCs cultured for 48 h. (d) Real-time PCR analyses of pcna, Cyclin A, and Cyclin E expression levels of control () and p57 knockdown (shp57, ) mESCs cultured for 48 h. (e, f) Western blot and related densitometric analysis of PCNA, Cyclin A, and Cyclin E protein levels of control and p57 knockdown (shp57) mESCs cultured for 48 h. (g) The growth curve of control () and p57-overexpressing (p57, ) mESCs. (h, i) EdU staining results of control () and p57-overexpressing (p57, ) mESCs cultured for 48 h. (j) Real-time PCR analyses of pcna, Cyclin A, and Cyclin E expression levels of control () and p57-overexpressing (p57, ) mESCs cultured for 48 h. (k, l) Western blot and related densitometric analysis of PCNA, Cyclin A, and Cyclin E protein levels of control and p57-overexpressing (p57) mESCs cultured for 48 h. μm. , , and .
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)
(i)
(j)
(k)
(l)