Research Article
p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation
Figure 4
p57 suppressed the proliferation of mESCs. (a) The growth curve of control () and p57 knockdown (shp57, ) mESCs. (b, c) EdU staining results of control () and p57 knockdown (shp57, ) mESCs cultured for 48 h. (d) Real-time PCR analyses of pcna, Cyclin A, and Cyclin E expression levels of control () and p57 knockdown (shp57, ) mESCs cultured for 48 h. (e, f) Western blot and related densitometric analysis of PCNA, Cyclin A, and Cyclin E protein levels of control and p57 knockdown (shp57) mESCs cultured for 48 h. (g) The growth curve of control () and p57-overexpressing (p57, ) mESCs. (h, i) EdU staining results of control () and p57-overexpressing (p57, ) mESCs cultured for 48 h. (j) Real-time PCR analyses of pcna, Cyclin A, and Cyclin E expression levels of control () and p57-overexpressing (p57, ) mESCs cultured for 48 h. (k, l) Western blot and related densitometric analysis of PCNA, Cyclin A, and Cyclin E protein levels of control and p57-overexpressing (p57) mESCs cultured for 48 h. μm. , , and .
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |
(i) |
(j) |
(k) |
(l) |