Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells

Figure 6

Functional analysis of the generated DPPSC-mediated hepatocyte-like cells. Time course hepatocyte enzymatic activities of (a) GGT, (b) AST, (c) APF, and (d) ALT were performed for the differentiated DPPSCs. A notable induction was observed at day 18. (e) CYP3A4 activity was measured by luminescence in DPPSCs at day 0 and day 22 of differentiation using rifampicin in the presence or absence of the antagonist erythromycin. (f) Alb secretion assay was performed at a time course differentiated DPPSCs; elevated level of secreted Alb was detected at day 19 and later. All measurements are relative to DPPSCs at day 0. and ^# relative to rifampicin treatment alone. Data are shown as the ().