METTL3-Mediated m⁶A RNA Modification Regulates Corneal Injury Repair

Figure 4

Methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) analysis of HCES3 cells with METTL3 knockdown. (a) Volcano map of differentially expressed genes. Red spot, genes that were significantly upregulated; green spot, genes that were significantly downregulated; and blue spot, genes that were not significantly downregulated. (b) Metagene profiles of m⁶A distribution across the transcriptome of HCES3 cells with METTL3 knockout. (c) Consensus motifs enriched with m⁶A peaks from transcripts identified in HCES3 cells with METTL3 knockout (2224 peaks). (d) GO analysis results of genes with m⁶A modifications. (e) The abundance of m⁶A in AHNAK and DDIT4 transcripts in METTL3 knockdown HCES3 cells was detected and mapped using Integrative Genomics Viewer (IGV). (f) GO mapping of genes with m⁶A modifications. (g) Western blot of gene expression in HCES3 cells after treatment with shMETTL3. (h) Gene expression (relative to GAPDH) quantified by RT-qPCR.

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