Research Article

miR-302d Competitively Binding with the lncRNA-341 Targets TLE4 in the Process of SSC Generation

Figure 2

miR-302d function verification in vitro. (a) Experimental scheme in vitro. (b) Cell morphology every two days, control: inducing by RA; miR-302d inhibitor: based on RA induction, miR-302d inhibitor lentivirus was infected on day 0; miR-302d mimics: based on RA induction, miR-302d mimics lentivirus was infected on day 0. (c) mRNA expression levels of marker genes were detected on day 4 and day 10; cvh and c-kit were used as PGC maker genes in chicken, integrin α6 and integrin β1 were SSC maker genes, and β-actin was the reference gene.
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