miR-302d Competitively Binding with the lncRNA-341 Targets TLE4 in the Process of SSC Generation

Figure 9

Wnt signaling pathway verification and ceRNA verification. (a) qRT-PCR result of related genes in Wnt signaling pathway of day 4 in vitro. (b) qRT-PCR result of day 10 in vitro. (c) qRT-PCR result of day 4.5 in vivo. (d) qRT-PCR result of day 18.5 in vivo. (e) Western blot test in vitro. (f) Western blot test in vivo. (g) lncRNA-341 expression test. (h) Dual-luciferase reporting assay; G1: pmiR-tle4-wt wild type; G2: pmiR-tle4-wt+miR-302d mimics; G3:pmiR-tle4-mt+miR-302d mimics; 3UTR-wt-miR: pmiR-report-wt+pRL-TK+miRNA-302d mimics; 3UTR-wt-miR-lnc341: pmiR-report-wt+pRL-TK+miRNA-302d mimics+pcDNA3.1-lnc341.

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