Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway

Figure 4

IGF-1 neutralizing antibody treatment. Mechanical stress in the nonstress group, the stress group, and the stress+10 μg/ml IGF-1 neutralizing antibody group. The IGF-1 neutralizing antibody reduced the mRNA levels of col-I (f) and col-III (g), but not IGF-1 (e). The IGF-1 neutralizing antibody also reduced the protein expression of IGF-1 (h), col-I (i), and col-III (j). In addition, the activation of pIGF-1R (a, b), pAKT (a, c), and pS6 (a, d) was reduced by the IGF-1 neutralizing antibody. Columns represent the of 3 samples, and each experiment was performed in triplicate. LFCs: ligamentum flavum cells; IGF-1: insulin-like growth factor 1; col-I: collagen I; col-III: collagen III. “” represents ; “ns” indicates .

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