Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway

Figure 6

Rapamycin treatment. Mechanical stress in the nonstress group, the stress group, and the stress+rapamycin group. Rapamycin reduced the mRNA levels of col-I (d) and col-III (e), but not IGF-1 (c). Rapamycin reduced the protein levels of col-I (g) and col-III (h), but not IGF-1 (f). In addition, rapamycin reduced the activation of pS6 (a, b). Columns represent the of 3 samples, and each experiment was performed in triplicate. LFCs: ligamentum flavum cells; IGF-1: insulin-like growth factor 1; col-I: collagen I; col-III: collagen III. “” represents ; “ns” indicates .

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