Research Article
Proteomic Analysis of Hypoxia-Induced Senescence of Human Bone Marrow Mesenchymal Stem Cells
Figure 3
Profiling of differentially expressed proteins between the hypoxia-induced group and the control group. (a) Statistical histogram of protein identification and quantification results by TMT. A total of 6824 proteins were identified, of which 6795 were quantified. (b) The up- (red) or downregulated (blue) proteins were displayed in different groups. 74 significantly differentially expressed proteins were identified, of which 50 were upregulated and 24 were downregulated after hypoxia induction for 12 hours (group C1), compared to hypoxia induction for 4 hours (group B1). 686 significantly differentially expressed proteins were identified, of which 400 were upregulated and 286 were downregulated after hypoxia induction for 12 hours (group C1), compared to the control group (group A1). 302 significantly differentially expressed proteins were identified, of which 227 were upregulated and 75 were downregulated after hypoxia induction for 4 hours (group B1), compared to the control group (group A1). (c) Volcano plot showing the up- (red) or downregulated (purple) proteins between the 12-hour hypoxia-induced group (group C1) and the control group (group A1). (d) The hierarchical clustering heat map of differentially expressed proteins between the 12-hour hypoxia-induced group (group C1) and the control group (group A1). Each column represents a set of samples, while each row represents a protein in the figure. The red represents significantly higher proteins, while purple represents significantly lower proteins in the heat map; the gray part means quantitative information without protein.
(a) |
(b) |
(c) |
(d) |