Neu5Ac Induces Human Dental Pulp Stem Cell Osteo-/Odontoblastic Differentiation by Enhancing MAPK/ERK Pathway Activation

<div>The effect of neuraminidase on DPSC osteo-/odontoblastic differentiation. (a) FITC-PNA lectin staining of DPSCs with or without neuraminidase (100 mU/ml) treatment. (b) CCK-8 assay results of neuraminidase-treated DPSC. (c) mRNA expression of DMP1, DSPP, BSP, and RUNX2 in DPSCs treated with neuraminidase (0, 1, 10, and 100 mU/ml) for 4 days. (d) Protein expression of DSPP, DMP1, and RUNX2 in DPSCs treated with neuraminidase (0, 1, 10, and 100 mU/ml) for 4 days. (e) ALP activity and Alizarin red staining after DPSCs were treated with neuraminidase (0, 1, 10, and 100 mU/ml). <svg height="9.5404pt" id="M17" style="vertical-align:-0.2324991pt" version="1.1" viewbox="-0.0498162 -9.3079 78.1489 9.5404" width="78.1489pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M409 504C401 567 396 607 392 642C354 654 312 665 266 665C137 665 60 583 60 487C60 374 161 325 225 290C300 250 355 215 355 141C355 68 311 21 235 21C131 21 86 122 71 183L41 176C48 128 61 42 68 21C78 16 93 8 118 0C142 -7 175 -15 216 -15C349 -15 438 69 438 174C438 287 344 333 265 374C186 414 138 449 138 522C138 576 172 631 249 631C336 631 363 562 380 499L409 504Z"></path></g><g transform="matrix(.013,0,0,-0.013,6.345,0)"><path d="M390 111C344 68 312 56 269 56C212 56 118 102 118 241C118 346 175 401 241 401C277 401 312 388 342 360C350 352 355 349 361 349C372 349 394 371 394 392C394 403 391 411 378 422C362 436 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Stem Cells International

fig2

Figure 2

Figure 2: Neu5Ac Induces Human Dental Pulp Stem Cell Osteo-/Odontoblastic Differentiation by Enhancing MAPK/ERK Pathway Activation 