Changes in Methylation Patterns of Tumor Suppressor Genes during Extended Human Embryonic Stem Cell Cultures

Table 1

Comparison of MS-MLPA, pyrosequencing, and real-time PCR data.

No.	Cell line	Gene	MS-MLPA	PSQ	Real-time PCR
1	CHA-15	CASP8	−	−	−
		FHIT	↑	↑	↓
2	CHA-20	CASP8	−	−	−
		FHIT	↓	↓	↑
3	CHA-31	CASP8	−	−	−
		FHIT	↑	↑	↓
4	CHA-32	CASP8	−	−	↓
		FHIT	↑	↑	↓
5	CHA-36	CASP8	↑	↑	(↑)
		FHIT	(↑)	↑	(↑)
6	CHA-40	CASP8	↑	↑	(↓)
		FHIT	↑	↑	↓
7	CHA-42	CASP8	↑	↑	↓
		FHIT	−	↓	↓
8	CHA-B3	CASP8	−	−	−
		FHIT	↓	(↓)	↑
9	H1	CASP8	−	−	−
		FHIT	↓	↓	↑
10	iPS (FS)-1	CASP8	(↑)	↑	↓
		FHIT	↑	↑	↓
		CHFR	↑	↑	↓

PSQ: pyrosequencing; ↑: increased in late passage than early passage; −: no change between early and late passages; ↓: decreased in late passage than early passage; (↑): showed a tendency to increase; (↓): showed a tendency to decrease.