Characterization and Study of Gene Expression Profiles of Human Periodontal Mesenchymal Stem Cells in Spheroid Cultures by Transcriptome Analysis

Figure 1

Gene expression profile in each individual sample analyzed by RNA-sequencing. RNA-seq detected 12,137 genes (), which were categorized into 3 patterns by hierarchical clustering approach (a; left). Cluster 1, which consists of genes upregulated in monolayer culture, and Cluster 3, which consists of genes upregulated in spheroid culture, were analyzed using DAVID to determine specific enriched GO terms. The figure shows the representative significantly enriched GO terms (Benjamini-) (a; right). Principal component analysis (PCA) was performed using 12,137-gene () expression datasets. The PCA plot shows two clearly distinguishable cell culture samples depicted as red (=monolayer) and blue (=spheroid) clusters (b).

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