Research Article
Characterization and Study of Gene Expression Profiles of Human Periodontal Mesenchymal Stem Cells in Spheroid Cultures by Transcriptome Analysis
Figure 3
Validation of NR4A2 expression in spheroid cultures of hPDLMSC. (a) Expression of NR4A2 mRNA in monolayer and spheroid-cultured hPDLMSC on day 3 by real-time RT-PCR. (compared with monolayer culture). (b) Expression of NR4A2 protein in monolayer and spheroid-cultured hPDLMSC on day 3 by western blotting. (c) NR4A1 and NR4A3 mRNA expression in monolayer and spheroid-cultured hPDLMSC on day 3 by real-time RT-PCR. (compared with monolayer culture). All experiments were performed with samples from four donors.
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