Research Article

Characterization and Study of Gene Expression Profiles of Human Periodontal Mesenchymal Stem Cells in Spheroid Cultures by Transcriptome Analysis

Figure 6

Effects of NR4A2 on chondrogenesis and adipogenesis in hPDLMSC spheroids and osteogenesis in monolayer-cultured hPDLMSCs. (a) Effects of siNR4A2 on CEBPα and PPARγ mRNA expression in monolayer-cultured hPDLMSCs with adipogenic differentiation medium. On day 7, the expression of CEBPα and PPARγ mRNA was examined by real-time RT-PCR. (b) Effects of siNR4A2 on COL2 and COL10 mRNA expression in monolayer- cultured hPDLMSCs with chondrogenic differentiation medium. On day 7, the expression of COL2 and COiL10 mRNA was examined by real time RT-PCR. (c) Confirmation of silencing of NR4A2 by siRNA in monolayer-cultured hPDLMSC. NR4A2 siRNA decreased mRNA expression of NR4A2. (d) Effects of siNR4A2 on Nanog and Oct4 mRNA expression in monolayer-cultured hPDLMSCs. The expression of NR4A1 and NR4A3 mRNA was examined by real-time RT-PCR. (e) Effects of siNR4A2 on ALP activity in monolayer-cultured hPDLMSCs cultured with OIM. On day 7, ALP activity was measured. All experiments were performed with samples from four donors.
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