Characterization and Study of Gene Expression Profiles of Human Periodontal Mesenchymal Stem Cells in Spheroid Cultures by Transcriptome Analysis

Table 1

Primer sequences for real-time RT-PCR.

Genes	Gene ID	Sequences of primers	PCR product
GAPDH	2597	F: 5-GAAGGTGAAGGTCGGAGTC-	206
		R: 3-GAAGATGGTGATGGGATTTC-5
ALP	249	F: 5-ACGTGGCTAAGAATGTCATC-3	457
		R: 3-CTGGTAGGCGATGTCCTTA-5
OPN	6696	F: 5-CCAAGTAAGTCCAACGAAAG-3	329
		R: 3-GGTGATGTCCTCGTCTGTA-5
COL1	1277	F: 5-AGGGCTCCAACGAGATCGAGATCCG-3	196
		R: 3-TACAGGAAGCAGACAGGGCCAACGTCG-5
RUNX2	860	F: 5-AACCCTTAATTTGCACTGGGTCA-3	120
		R: 3-CAAATTCCAGCAATGTTTGTGCTAC-5
NR4A1	3164	F: 5-AAGCCACATTGTTGCCAAGACCTG-3	122
		R: 3-TGGTGTCCCATATTGGGCTTGGAT-5
NR4A2	4929	F: 5-GTCTCAGCTGCTCGACACG-3	139
		R: 3-TTTTGCACTGTGCGCTTAAA-5
NR4A3	8013	F: 5-TACACCAAGCTGACCATGGACCTT-3	110
		R: 3-ATTTGGTACACGCAGGAAGGCTTG-5
OCT4	5460	F: 5-AGCAAAACCCGGAGGAGT-3	93
		R: 3-CCACATCGGCCTGTGTATATC-5
NANOG	79923	F: 5-TGAACCTCAGCTACAAACAG-3	134
		R: 3-TGGTGGTAGGAAGAGTAAAG-5
SOD2	6648	F: 5-TTCTGGACAAACCTCAGCCC-3	49
		R: 3-CGTTTGATGGCTTCCAGCA-5
CEBPA	1050	F: 5-AGGAGGATGAAGCCAAGCAGCT-3	113
		R: 3-AGTGCGCGATCTGGAACTGCAG-5
PPARG	5468	F: 5-AGCCTGCGAAAGCCTTTTGGTG-3	129
		R: 3-GGCTTCACATTCAGCAAACCTGG-5
COL2	1280	F: 5-CCTGGCAAAGATGGTGAGACAG-3	127
		R: 3-CCTGGTTTTCCACCTTCACCTG-5
COL10	1300	F: 5-CGCTGAACGATACCAAATGCCC-3	108
		R: 3-TGGACCAGGAGTACCTTGCTCT-5

Annealing temperature 60°C, cycle number 40.