Hydrostatic Pressure Modulates Intervertebral Disc Cell Survival and Extracellular Matrix Homeostasis via Regulating Hippo-YAP/TAZ Pathway

Figure 7

Effect of hydrostatic pressure on YAP/TAZ pathway-mediated apoptosis of the rabbit notochordal NP and fibrocartilaginous inner AF: (a) YAP/TAZ complex (green) fluorescent staining of the fibrocartilaginous inner AF under low-loading (0.5 MPa) or high-magnitude (1.0 MPa) physiological hydrostatic pressure (200x); (b) statistic analysis of YAP/TAZ complex nucleus/cytoplasm distribution; (c) western blotting analysis of the expression levels of N-CDH, integrin β1, and YAP/TAZ-mediated apoptosis markers (YAP/nucleus, YAP/cytoplasm, Caspase3) of the notochordal NP under graded hydrostatic pressure; (d) western blotting analysis of the expression levels of N-CDH, integrin β1, and YAP/TAZ-mediated apoptosis markers (YAP/nucleus, YAP/cytoplasm, Caspase3) of the fibrocartilaginous inner AF under graded hydrostatic pressure; (e) statistic analysis of the western blots of the notochordal NP under graded hydrostatic pressure; (f) statistic analysis of the western blots of the fibrocartilaginous inner AF under graded hydrostatic pressure. . ^#.  μm.

(a)

(b)

(c)

(d)

(e)

(f)