MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1

Figure 2

Transfection efficiency and the effect of hsa-let-7b on the proliferation of PDLSCs. (a) The expression of hsa-let-7b during the osteogenic differentiation of PDLSCs for 0, 3, and 7, days, respectively. . (b) Transfection efficacy of hsa-let-7b was measured at day 3 by qRT-PCR. (c) CCK-8 analysis showed that there was no significant difference between mimic and mimic NC at 0, 1, 3, 5, and 7, respectively. . (d) CCK-8 analysis showed that there was no significant difference between inhibitor and inhibitor NC at 0, 1, 3, 5, and 7, respectively. . (e, f) Cell proliferation of PDLSCs was detected by EdU staining and assay. . EdU: 5-ethynyl-2-deoxyuridine; mimic: hsa-let-7b mimics; mimic NC: mimics control; inhibitor: hsa-let-7b inhibitor; inhibitor NC: inhibitor control.

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