MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1

Figure 6

CTHRC1 enhanced the osteogenic differentiation capacity of PDLSCs. (a) The result of qRT-PCR showed that transfection effect of Si-2 was better. . (b) QRT-PCR showed the expression of osteogenesis-related genes (ALP, RUNX2, and OSX) downregulated. . (c) Western blot analysis of osteogenic markers in PDLSCs transfected with Si-CTHRC1. (d) Relative grayscale analyses of (c). , . (e, f) 7 days after mineralization, ALP staining and ALP activity analysis were performed (). . (g, h) Results of ARS staining and CPC assay at day 14 (). . (i, j) Immunofluorescence assay revealed that the protein expression of RUNX2 and ALP was downregulated in Si-CTHRC1 group as compared with controls ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)