MicroRNA Hsa-Let-7b Regulates the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting CTHRC1

Figure 7

CTHRC1 partially reverses the influence of hsa-let-7b on the osteogenic differentiation of PDLSCs. (a, b) ALP staining and ALP activity analysis on day 7 in four group (). . (c, d) ARS staining and relative CPC assay were performed after osteogenic inducting 14 days (). . (e) Western blot analysis of ALP, RUNX2, and OSX. (f) Grayscale analyses of (c). . (g) Relative mRNA expression of osteogenic genes. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)