Inhibition of Endoplasmic Reticulum Stress by 4-Phenyl Butyric Acid Presents Therapeutic Effects on Periodontitis: Experimental Studies In Vitro and in Rats

Figure 2

4-PBA attenuates inflammatory response of P-PDLSCs and LPS-treated H-PDLSCs. The secretion of proinflammatory factors TNF-α, IL-1β, and IL-6 was detected by using ELISA in (a) P-PDLSCs treated without or with 5 mM 4-PBA and (b) H-PDLSCs treated in the absence or presence of 10 mg/l LPS or LPS in association with 5 mM 4-PBA. Western blot analysis showed the expression levels of p-NF-κB and NF-κB, as well as the ratio of p-NF-κB/NF-κB in (c) P-PDLSCs treated without or with 5 mM 4-PBA or in (d) H-PDLSCs treated in the absence or presence of 10 mg/l LPS or LPS in association with 5 mM 4-PBA. Internal control used β-actin. (e, f) Cell viability was determined by using CCK-8 assay after two days treatment. The data represent mean ± standard deviations (). , , .

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