A Two-Stage Process for Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells into Neuronal-like Cells

<div>Gene expression profile of WJ-MSCs primed neuroectodermal cells. Panel A: expression profile of MSC-specific genes. Panel B: expression profile (delta Ct) of <i>SOX1</i> in WJ-MSCs and in primed neuroectodermal cells in all three-differentiation condition. “<b>###</b>” indicates no amplification observed in WJ-MSCs. Panel C: expression profile of neural progenitor-specific transcription factors. Panel D: expression profile of neural stem cell-specific genes. Panel E: expression profile of neuronal receptor-specific genes. Panel F: expression profile of neuron-specific transcription factors. <i>GAPDH</i> was used as normalization control. Bar 1 (cells induced with FGF2 and EGF), Bar 2 (cells induced with FGF2, EGF, and NGF), and Bar 3 (cells induced with FGF2, EGF, and PDGF-BB). Relative fold changes of gene expressions are shown as compared to that in WJ-MSCs. 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Stem Cells International

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Figure 4

Figure 4: A Two-Stage Process for Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells into Neuronal-like Cells 