IL-1 Receptor Antagonist Protects the Osteogenesis Capability of Gingival-Derived Stem/Progenitor Cells under Inflammatory Microenvironment Induced by Porphyromonas gingivalis Lipopolysaccharides

Table 1

Primer sequences for RT-PCR (supplied by Roche).

Gene	Forward primer (5-3)	Reverse primer (5-3)
ALP	GAGGAACGGATCTCGGGGTA	ATGAGTTGGTAAGGCAGGGTC
OPN	TGAGTCTGGAAATAACTAATGTGTTTGA	GAACATAGACATAACCCTGAAGCTTTT
OCN	TATGGCACCACCGTTTAGGG	CTGTGCCGTCCATACTTTCG
COL-I	ACGCTCAAGTCGCTGAACAA	TCAATCCAGTAGTCTCCGCTCT
Runx2	CAACCACAGAACCACAAGTGC	CACTGACTCGGTTGGTCTCG
TLR4	GAGGCAGCAGGTCGAATTGT	AGAAGATGTGCCTCCCCAGA
IκBα	AACAGTCTGAACTCGCCACC	CACCAACCGCTCCTTCTTGA
β-Actin	AGTACAACCTTCTTGCAGCTCCTC	TGCCGGAGCCGTTGTCG

ALP: alkaline phosphatase; OPN: osteopontin; OCN: osteocalcin; COL-I: type I collagen; RNX2: runt-related transcription factor 2; TLR4: toll-like receptor 4; IκBα: inhibitor of nuclear factor kappa-B alpha.