CGF Membrane Promotes Periodontal Tissue Regeneration Mediated by hUCMSCs through Upregulating TAZ and Osteogenic Differentiation Genes

Figure 4

The effects of overexpressed or silenced TAZ on the expressions of osteoblast differentiation, osteogenesis-related genes, and TAZ. (a) Alizarin staining was used to identify the differentiation of stem cells into osteoblasts, under 200 × magnification. Mineral accumulation was quantified. (b–d) The mRNA expressions of osteoblast-related genes were analyzed by qRT-PCR. β-Actin was employed as an internal control. (e and f) TAZ protein expression in cytoplasm and nucleus was detected by Western blot. β-Actin and Lamin B1 were employed as internal controls. All experiments were performed in independent triplicate. , vs. vector; ^#, ^##, ^### vs. CGF + vector; ^{^}, ^{^^} vs. shTAZ; ^&, ^&& vs. pc-TAZ.

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